LeHavreV1, Main, Exploration, bibRecord, 000062

First evidence for a Vibrio strain pathogenic to Mytilus edulis altering hemocyte immune capacities.

Identifieur interne : 000062 ( Main/Exploration ); précédent : 000061; suivant : 000063

First evidence for a Vibrio strain pathogenic to Mytilus edulis altering hemocyte immune capacities.

Auteurs : Yosra Ben Cheikh [France] ; Marie-Agnès Travers [France] ; Benjamin Morga [France] ; Yoann Godfrin [France] ; Damien Rioult [France] ; Frank Le Foll [France]

Source :

Developmental and comparative immunology [ 1879-0089 ] ; 2016.

RBID : pubmed:26719026

English descriptors

KwdEn :
- Animals, Cell Movement, Hemocytes (immunology), Hemocytes (microbiology), Immune Evasion, Immunity, Cellular, Immunity, Innate, Immunomodulation, Mytilus edulis (immunology), Oxidative Stress, Phagocytosis, Species Specificity, Vibrio (immunology), Vibrio (pathogenicity), Vibrio Infections (immunology), Virulence.
MESH :
- immunology : Hemocytes, Mytilus edulis, Vibrio, Vibrio Infections.
- microbiology : Hemocytes.
- pathogenicity : Vibrio.
- Animals, Cell Movement, Immune Evasion, Immunity, Cellular, Immunity, Innate, Immunomodulation, Oxidative Stress, Phagocytosis, Species Specificity, Virulence.

Abstract

Bacterial isolates were obtained from mortality events affecting Mytilus edulis and reported by professionals in 2010-2013 or from mussel microflora. Experimental infections allowed the selection of two isolates affiliated to Vibrio splendidus/Vibrio hemicentroti type strains: a virulent 10/068 1T1 (76.6% and 90% mortalities in 24 h and 96 h) and an innocuous 12/056 M24T1 (0% and 23.3% in 24 h and 96 h). These two strains were GFP-tagged and validated for their growth characteristics and virulence as genuine models for exposure. Then, host cellular immune responses to the microbial invaders were assessed. In the presence of the virulent strain, hemocyte motility was instantaneously enhanced but markedly slowed down after 2 h exposure. By contrast, hemocyte velocity increased in the presence of the innocuous 12/056 M24T1. At the same time interval, 10/068 1T1 invaded hemocytes and was more rapidly internalized than the innocuous strain. Extracellular products (ECPs) prepared from 10/068 1T1 cultures significantly inhibited phagocytic activity while 12/056 M24T1 ECPs had no effect. Furthermore, the pathogenic strain and its ECPs inhibited oxidative burst unlike 12/056 M24T1 strain/ECPs which enhanced ROS production. Taken together, our results suggest that the mussel pathogen 10/068 1T1 may escape immune response by altering hemocytes functions.

DOI: 10.1016/j.dci.2015.12.014
PubMed: 26719026

Affiliations:

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">First evidence for a Vibrio strain pathogenic to Mytilus edulis altering hemocyte immune capacities.</title>
<author><name sortKey="Ben Cheikh, Yosra" sort="Ben Cheikh, Yosra" uniqKey="Ben Cheikh Y" first="Yosra" last="Ben Cheikh">Yosra Ben Cheikh</name>
<affiliation wicri:level="3"><nlm:affiliation>Laboratory of Ecotoxicology- Aquatic Environments, UMR-I 02, SEBIO, University of Le Havre, F-76063, Le Havre Cedex, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Laboratory of Ecotoxicology- Aquatic Environments, UMR-I 02, SEBIO, University of Le Havre, F-76063, Le Havre Cedex</wicri:regionArea>
<placeName><region type="region" nuts="2">Région Normandie</region>
<region type="old region" nuts="2">Haute-Normandie</region>
<settlement type="city">Le Havre</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Travers, Marie Agnes" sort="Travers, Marie Agnes" uniqKey="Travers M" first="Marie-Agnès" last="Travers">Marie-Agnès Travers</name>
<affiliation wicri:level="3"><nlm:affiliation>Ifremer, SG2M-LGPMM, Laboratoire de Génétique et Pathologie des Mollusques Marins, Avenue de Mus de Loup, 17390, La Tremblade, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Ifremer, SG2M-LGPMM, Laboratoire de Génétique et Pathologie des Mollusques Marins, Avenue de Mus de Loup, 17390, La Tremblade</wicri:regionArea>
<placeName><region type="region" nuts="2">Nouvelle-Aquitaine</region>
<region type="old region" nuts="2">Poitou-Charentes</region>
<settlement type="city">La Tremblade</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Morga, Benjamin" sort="Morga, Benjamin" uniqKey="Morga B" first="Benjamin" last="Morga">Benjamin Morga</name>
<affiliation wicri:level="3"><nlm:affiliation>Ifremer, SG2M-LGPMM, Laboratoire de Génétique et Pathologie des Mollusques Marins, Avenue de Mus de Loup, 17390, La Tremblade, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Ifremer, SG2M-LGPMM, Laboratoire de Génétique et Pathologie des Mollusques Marins, Avenue de Mus de Loup, 17390, La Tremblade</wicri:regionArea>
<placeName><region type="region" nuts="2">Nouvelle-Aquitaine</region>
<region type="old region" nuts="2">Poitou-Charentes</region>
<settlement type="city">La Tremblade</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Godfrin, Yoann" sort="Godfrin, Yoann" uniqKey="Godfrin Y" first="Yoann" last="Godfrin">Yoann Godfrin</name>
<affiliation wicri:level="3"><nlm:affiliation>Ifremer, SG2M-LGPMM, Laboratoire de Génétique et Pathologie des Mollusques Marins, Avenue de Mus de Loup, 17390, La Tremblade, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Ifremer, SG2M-LGPMM, Laboratoire de Génétique et Pathologie des Mollusques Marins, Avenue de Mus de Loup, 17390, La Tremblade</wicri:regionArea>
<placeName><region type="region" nuts="2">Nouvelle-Aquitaine</region>
<region type="old region" nuts="2">Poitou-Charentes</region>
<settlement type="city">La Tremblade</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Rioult, Damien" sort="Rioult, Damien" uniqKey="Rioult D" first="Damien" last="Rioult">Damien Rioult</name>
<affiliation wicri:level="3"><nlm:affiliation>Laboratory of Ecotoxicology- Aquatic Environments, UMR-I 02, SEBIO, University of Reims Champagne Ardenne, Campus Moulin de la House, F-51100, Reims, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Laboratory of Ecotoxicology- Aquatic Environments, UMR-I 02, SEBIO, University of Reims Champagne Ardenne, Campus Moulin de la House, F-51100, Reims</wicri:regionArea>
<placeName><region type="region" nuts="2">Grand Est</region>
<region type="old region" nuts="2">Champagne-Ardenne</region>
<settlement type="city">Reims</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Le Foll, Frank" sort="Le Foll, Frank" uniqKey="Le Foll F" first="Frank" last="Le Foll">Frank Le Foll</name>
<affiliation wicri:level="3"><nlm:affiliation>Laboratory of Ecotoxicology- Aquatic Environments, UMR-I 02, SEBIO, University of Le Havre, F-76063, Le Havre Cedex, France. Electronic address: frank.lefoll@univ-lehavre.fr.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Laboratory of Ecotoxicology- Aquatic Environments, UMR-I 02, SEBIO, University of Le Havre, F-76063, Le Havre Cedex</wicri:regionArea>
<placeName><region type="region" nuts="2">Région Normandie</region>
<region type="old region" nuts="2">Haute-Normandie</region>
<settlement type="city">Le Havre</settlement>
</placeName>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2016">2016</date>
<idno type="RBID">pubmed:26719026</idno>
<idno type="pmid">26719026</idno>
<idno type="doi">10.1016/j.dci.2015.12.014</idno>
<idno type="wicri:Area/PubMed/Corpus">000047</idno>
<idno type="wicri:Area/PubMed/Curation">000047</idno>
<idno type="wicri:Area/PubMed/Checkpoint">000047</idno>
<idno type="wicri:Area/Ncbi/Merge">000517</idno>
<idno type="wicri:Area/Ncbi/Curation">000517</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">000517</idno>
<idno type="wicri:Area/Main/Merge">000061</idno>
<idno type="wicri:Area/Main/Curation">000062</idno>
<idno type="wicri:Area/Main/Exploration">000062</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">First evidence for a Vibrio strain pathogenic to Mytilus edulis altering hemocyte immune capacities.</title>
<author><name sortKey="Ben Cheikh, Yosra" sort="Ben Cheikh, Yosra" uniqKey="Ben Cheikh Y" first="Yosra" last="Ben Cheikh">Yosra Ben Cheikh</name>
<affiliation wicri:level="3"><nlm:affiliation>Laboratory of Ecotoxicology- Aquatic Environments, UMR-I 02, SEBIO, University of Le Havre, F-76063, Le Havre Cedex, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Laboratory of Ecotoxicology- Aquatic Environments, UMR-I 02, SEBIO, University of Le Havre, F-76063, Le Havre Cedex</wicri:regionArea>
<placeName><region type="region" nuts="2">Région Normandie</region>
<region type="old region" nuts="2">Haute-Normandie</region>
<settlement type="city">Le Havre</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Travers, Marie Agnes" sort="Travers, Marie Agnes" uniqKey="Travers M" first="Marie-Agnès" last="Travers">Marie-Agnès Travers</name>
<affiliation wicri:level="3"><nlm:affiliation>Ifremer, SG2M-LGPMM, Laboratoire de Génétique et Pathologie des Mollusques Marins, Avenue de Mus de Loup, 17390, La Tremblade, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Ifremer, SG2M-LGPMM, Laboratoire de Génétique et Pathologie des Mollusques Marins, Avenue de Mus de Loup, 17390, La Tremblade</wicri:regionArea>
<placeName><region type="region" nuts="2">Nouvelle-Aquitaine</region>
<region type="old region" nuts="2">Poitou-Charentes</region>
<settlement type="city">La Tremblade</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Morga, Benjamin" sort="Morga, Benjamin" uniqKey="Morga B" first="Benjamin" last="Morga">Benjamin Morga</name>
<affiliation wicri:level="3"><nlm:affiliation>Ifremer, SG2M-LGPMM, Laboratoire de Génétique et Pathologie des Mollusques Marins, Avenue de Mus de Loup, 17390, La Tremblade, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Ifremer, SG2M-LGPMM, Laboratoire de Génétique et Pathologie des Mollusques Marins, Avenue de Mus de Loup, 17390, La Tremblade</wicri:regionArea>
<placeName><region type="region" nuts="2">Nouvelle-Aquitaine</region>
<region type="old region" nuts="2">Poitou-Charentes</region>
<settlement type="city">La Tremblade</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Godfrin, Yoann" sort="Godfrin, Yoann" uniqKey="Godfrin Y" first="Yoann" last="Godfrin">Yoann Godfrin</name>
<affiliation wicri:level="3"><nlm:affiliation>Ifremer, SG2M-LGPMM, Laboratoire de Génétique et Pathologie des Mollusques Marins, Avenue de Mus de Loup, 17390, La Tremblade, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Ifremer, SG2M-LGPMM, Laboratoire de Génétique et Pathologie des Mollusques Marins, Avenue de Mus de Loup, 17390, La Tremblade</wicri:regionArea>
<placeName><region type="region" nuts="2">Nouvelle-Aquitaine</region>
<region type="old region" nuts="2">Poitou-Charentes</region>
<settlement type="city">La Tremblade</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Rioult, Damien" sort="Rioult, Damien" uniqKey="Rioult D" first="Damien" last="Rioult">Damien Rioult</name>
<affiliation wicri:level="3"><nlm:affiliation>Laboratory of Ecotoxicology- Aquatic Environments, UMR-I 02, SEBIO, University of Reims Champagne Ardenne, Campus Moulin de la House, F-51100, Reims, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Laboratory of Ecotoxicology- Aquatic Environments, UMR-I 02, SEBIO, University of Reims Champagne Ardenne, Campus Moulin de la House, F-51100, Reims</wicri:regionArea>
<placeName><region type="region" nuts="2">Grand Est</region>
<region type="old region" nuts="2">Champagne-Ardenne</region>
<settlement type="city">Reims</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Le Foll, Frank" sort="Le Foll, Frank" uniqKey="Le Foll F" first="Frank" last="Le Foll">Frank Le Foll</name>
<affiliation wicri:level="3"><nlm:affiliation>Laboratory of Ecotoxicology- Aquatic Environments, UMR-I 02, SEBIO, University of Le Havre, F-76063, Le Havre Cedex, France. Electronic address: frank.lefoll@univ-lehavre.fr.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Laboratory of Ecotoxicology- Aquatic Environments, UMR-I 02, SEBIO, University of Le Havre, F-76063, Le Havre Cedex</wicri:regionArea>
<placeName><region type="region" nuts="2">Région Normandie</region>
<region type="old region" nuts="2">Haute-Normandie</region>
<settlement type="city">Le Havre</settlement>
</placeName>
</affiliation>
</author>
</analytic>
<series><title level="j">Developmental and comparative immunology</title>
<idno type="eISSN">1879-0089</idno>
<imprint><date when="2016" type="published">2016</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term>
<term>Cell Movement</term>
<term>Hemocytes (immunology)</term>
<term>Hemocytes (microbiology)</term>
<term>Immune Evasion</term>
<term>Immunity, Cellular</term>
<term>Immunity, Innate</term>
<term>Immunomodulation</term>
<term>Mytilus edulis (immunology)</term>
<term>Oxidative Stress</term>
<term>Phagocytosis</term>
<term>Species Specificity</term>
<term>Vibrio (immunology)</term>
<term>Vibrio (pathogenicity)</term>
<term>Vibrio Infections (immunology)</term>
<term>Virulence</term>
</keywords>
<keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Hemocytes</term>
<term>Mytilus edulis</term>
<term>Vibrio</term>
<term>Vibrio Infections</term>
</keywords>
<keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Hemocytes</term>
</keywords>
<keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Vibrio</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Cell Movement</term>
<term>Immune Evasion</term>
<term>Immunity, Cellular</term>
<term>Immunity, Innate</term>
<term>Immunomodulation</term>
<term>Oxidative Stress</term>
<term>Phagocytosis</term>
<term>Species Specificity</term>
<term>Virulence</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Bacterial isolates were obtained from mortality events affecting Mytilus edulis and reported by professionals in 2010-2013 or from mussel microflora. Experimental infections allowed the selection of two isolates affiliated to Vibrio splendidus/Vibrio hemicentroti type strains: a virulent 10/068 1T1 (76.6% and 90% mortalities in 24 h and 96 h) and an innocuous 12/056 M24T1 (0% and 23.3% in 24 h and 96 h). These two strains were GFP-tagged and validated for their growth characteristics and virulence as genuine models for exposure. Then, host cellular immune responses to the microbial invaders were assessed. In the presence of the virulent strain, hemocyte motility was instantaneously enhanced but markedly slowed down after 2 h exposure. By contrast, hemocyte velocity increased in the presence of the innocuous 12/056 M24T1. At the same time interval, 10/068 1T1 invaded hemocytes and was more rapidly internalized than the innocuous strain. Extracellular products (ECPs) prepared from 10/068 1T1 cultures significantly inhibited phagocytic activity while 12/056 M24T1 ECPs had no effect. Furthermore, the pathogenic strain and its ECPs inhibited oxidative burst unlike 12/056 M24T1 strain/ECPs which enhanced ROS production. Taken together, our results suggest that the mussel pathogen 10/068 1T1 may escape immune response by altering hemocytes functions.</div>
</front>
</TEI>
<affiliations><list><country><li>France</li>
</country>
<region><li>Champagne-Ardenne</li>
<li>Grand Est</li>
<li>Haute-Normandie</li>
<li>Nouvelle-Aquitaine</li>
<li>Poitou-Charentes</li>
<li>Région Normandie</li>
</region>
<settlement><li>La Tremblade</li>
<li>Le Havre</li>
<li>Reims</li>
</settlement>
</list>
<tree><country name="France"><region name="Région Normandie"><name sortKey="Ben Cheikh, Yosra" sort="Ben Cheikh, Yosra" uniqKey="Ben Cheikh Y" first="Yosra" last="Ben Cheikh">Yosra Ben Cheikh</name>
</region>
<name sortKey="Godfrin, Yoann" sort="Godfrin, Yoann" uniqKey="Godfrin Y" first="Yoann" last="Godfrin">Yoann Godfrin</name>
<name sortKey="Le Foll, Frank" sort="Le Foll, Frank" uniqKey="Le Foll F" first="Frank" last="Le Foll">Frank Le Foll</name>
<name sortKey="Morga, Benjamin" sort="Morga, Benjamin" uniqKey="Morga B" first="Benjamin" last="Morga">Benjamin Morga</name>
<name sortKey="Rioult, Damien" sort="Rioult, Damien" uniqKey="Rioult D" first="Damien" last="Rioult">Damien Rioult</name>
<name sortKey="Travers, Marie Agnes" sort="Travers, Marie Agnes" uniqKey="Travers M" first="Marie-Agnès" last="Travers">Marie-Agnès Travers</name>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Wicri/France/explor/LeHavreV1/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000062 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000062 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Wicri/France
   |area=    LeHavreV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     pubmed:26719026
   |texte=   First evidence for a Vibrio strain pathogenic to Mytilus edulis altering hemocyte immune capacities.
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i   -Sk "pubmed:26719026" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd   \
       | NlmPubMed2Wicri -a LeHavreV1

This area was generated with Dilib version V0.6.25.
Data generation: Sat Dec 3 14:37:02 2016. Site generation: Tue Mar 5 08:25:07 2024

Serveur d'exploration sur la visibilité du Havre

First evidence for a Vibrio strain pathogenic to Mytilus edulis altering hemocyte immune capacities.

First evidence for a Vibrio strain pathogenic to Mytilus edulis altering hemocyte immune capacities.

Source :

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

	Serveur d'exploration sur la visibilité du Havre
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.